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KMID : 0380219930260020118
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Journal of Biochemistry and Molecular Biology
1993 Volume.26 No. 2 p.118 ~ p.124
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Development and Characterization of Monoclonal Antilbodies Recognizing Deoxyribonucleoside Derivatives
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Eun-Mi Choi
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Abstract
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A purine deoxyriboside (6-thiopurine-2'-deoxyriboside, dPu-SH) and a pyrimidine deoxyriboside (4-thio-2'-deoxyuridine, dU-SH) were conjugated to bovine serum albumin (BSA) in the way that dexyribose rings were exposed by linking C-6 of
purine
ring and C-4 of pyrimidine ring to the protein, respectively. Monoclonal antibodies were produced by using these two conjugates as antigens. Group I monoclonal antibodies (1B2, 2A3, 4D4, and 8B6) from dPu-BSA immunized mouse showed
affinity for
purine deoxyriboside derivatives with substitution on N-1, C-2, and C-6 but showed no affinity for the derivatives with N-7 and C-8 substitution. Group II monoclonal antibodies (3A3, 3A5, 6A2, and 9B1) from dU-BSA immunized mouse
showed
affinity for pyrimidine deoxyribosides with substitution on C-2, C-4, and C-5, None of the antibodies had affinity for base dervatives, deoxyribose, and ribonucleosides. It suggests that these monoclonal antibodies may be
useful
for the study of mechanisms of DNA damage by facilitating isolation of various deoxyribonucleosides excised from damaged DNA.
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